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Published on: May 30, 2013
Degradation of cholesterol by Bacillus subtilis SFF34 isolated from Korean traditional fermented flatfish
1Department of Food Science and Technology, Kyungpook National University, Taegu, Korea.
Aims:
To examine cholesterol degradation by Bacillus subtilis SFF34.
Methods And Results:
Cholesterol degradation and cholesterol oxidase production by B. subtilis SFF34 were investigated in a medium containing 0.2% cholesterol. In addition, the oxidized product of cholesterol by the purified cholesterol oxidase was detected using a gas chromatograph. Cholesterol oxidase production reached its maximal level (3.14 U ml(-1) after 24 h of incubation in the cholesterol medium. The residual cholesterol content reduced to 0.98 mg g(-1) after 60 h of cultivation in the cholesterol medium. Two cholesterol oxidases were purified from the culture supernatant fluid and their reaction product against cholesterol was identified as 4-cholesten-3-one.
Conclusions:
B. subtilis SFF34 degraded cholesterol and produced a high level of extracellular cholesterol oxidase.
Significance And Impact Of The Study:
Bacillus subtilis will be very useful for the reduction of cholesterol in many fermented foods and as a source of cholesterol oxidase.
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