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Updated: Aug 12, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
Published on: August 17, 2015
Prediction of chromatographic retention and protein identification in liquid chromatography/mass spectrometry
Magnus Palmblad1, Margareta Ramström, Karin E Markides
1The Angstrom Laboratory, Division of Ion Physics, Box 534, and Institute of Chemistry, Department of Analytical Chemistry, Box 531, Uppsala University, SE-751 21 Uppsala, Sweden. palmblad1@llnl.gov
Abstract:
Liquid chromatography coupled on- or off-line with mass spectrometry is rapidly advancing as a tool in proteomics capable of dealing with the inherent complexity in biology and complementing conventional approaches based on two-dimensional gel electrophoresis. Proteins can be identified by proteolytic digestion and peptide mass fingerprinting or by searching databases using short-sequence tags generated by tandem mass spectrometry. This paper shows that information on the chromatographic behavior of peptides can assist protein identification by peptide mass fingerprinting in liquid chromatography/mass spectrometry. This additional information is significant and already available at no extra experimental cost.
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