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Related Experiment Video

Updated: Jan 9, 2026

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

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Published on: March 19, 2018

Epimorphin expression in intestinal myofibroblasts induces epithelial morphogenesis.

Christine Fritsch¹, Elzbieta A Swietlicki, Olivier Lefebvre

¹Division of Gastroenterology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA.

The Journal of Clinical Investigation

|December 5, 2002

Summary

Mesenchymal epimorphin is crucial for developing the gut

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Area of Science:

Developmental Biology
Gastroenterology
Cell Biology

Background:

Gut development relies on endoderm-mesenchyme interactions.
Epimorphin, a mesenchymal protein, is present during fetal gut development.

Purpose of the Study:

To investigate the role of epimorphin in gut ontogeny and crypt-villus axis formation.

Main Methods:

Transfected rat intestinal myofibroblasts with epimorphin cDNA (sense and antisense).
Cocultured myofibroblasts with Caco2 intestinal cells.
Implanted rat gut endoderm with transfected myofibroblasts into chick embryos.

Main Results:

Epimorphin overexpression in myofibroblasts induced Caco2 cells to form compact clusters.
In vivo grafts with epimorphin overexpression showed well-formed villi and crypts.
Epimorphin increased bone morphogenetic protein 4 (Bmp4) expression, and noggin partially blocked its effects.

Conclusions:

Mesenchymal epimorphin is essential for proper crypt-villus morphogenesis.
Epimorphin's effects are mediated by secreted factors, including Bmp4.