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Related Experiment Videos

Plastid and stromule morphogenesis in tomato.

Kevin A Pyke1, Caroline A Howells

  • 1Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington campus, Loughborough, Leicestershire LE12 5RD, UK. kevin.pyke@nottingham.ac.uk

Annals of Botany
|December 6, 2002
PubMed
Summary

Green fluorescent protein reveals novel plastid stromule structures in tomato plants. These findings suggest potential new pathways for carotenoid biosynthesis and vesicle secretion in tomato fruit and glandular trichomes.

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Area of Science:

  • Plant Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Plastids are vital organelles in plant cells, involved in photosynthesis and biosynthesis.
  • Stromules are dynamic extensions of plastids, but their function and morphology remain incompletely understood.
  • Tomato (Lycopersicon esculentum Mill.) offers a model system for studying fruit development and pigment biosynthesis.

Purpose of the Study:

  • To visualize and characterize the morphology of plastids and stromules in various tomato tissues using a fluorescent protein marker.
  • To investigate the structure of chromoplasts in tomato fruit pericarp cells.
  • To explore potential novel functions of stromules and plastids in tomato.

Main Methods:

  • Expression of green fluorescent protein targeted to plastids in tomato (Lycopersicon esculentum Mill.).

Related Experiment Videos

  • Microscopic analysis of plastid and stromule morphology in epidermal cells, trichomes (stems and petioles), and fruit pericarp.
  • Observation of chromoplasts and their interconnections in mature tomato fruit.

    • Main Results:

    • Detailed morphology of plastids and stromules in tomato stems, petioles, and fruit pericarp was revealed.
    • A novel finding includes extensive bead-like structures along stromules, often appearing as free vesicles.
    • Interconnections between chromoplasts were frequently observed in fruit pericarp cells, suggesting a potential network structure.

    Conclusions:

    • The observed beaded stromules and free vesicles may represent a novel secretory mechanism for plastids in glandular trichomes.
    • The interconnected chromoplast network in fruit pericarp suggests implications for efficient carotenoid biosynthesis.
    • Further research is warranted to elucidate the precise roles of these structures in tomato physiology and development.