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[Changes of the mRNA expression and the intestinal mucosal cyclooxygenase activity in scalded rats]
1Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing, 400038 P.R. China.
Objective:
To investigate the changes of the mRNA expression and the intestinal mucosal cyclooxygenase (COXs) activity in scalded rats.
Methods:
Wistar rats inflicted with 30% TBSA III degree scalding were employed as the model. The changes of COX-1, COX-2 activities were determined by substrate fluorescence analysis and the mRNA expressions of COX-1 and COX-2 by RT-PCR.
Results:
The mRNA expressions and the activities of COX-2 in rat intestinal mucosa increased obviously after injury. But those of COX-1 exhibited lower range of change.
Conclusion:
The pathological mechanism of rat intestinal mucosa injury after scalding might be closely related to the COXs participation by different styles between the two enzymes.
