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Comparison of three methods for plasma fibrinogen
Annals of Clinical and Laboratory Science
|January 1, 1976
Summary
Three plasma fibrinogen measurement methods were evaluated for accuracy and precision. These assays, including clottable protein, clot absorbance, and thrombin time, demonstrate good correlation and reliability in normal and heparinized samples.
Area of Science:
- Clinical Chemistry
- Hematology
- Biochemical Assays
Background:
- Fibrinogen is a critical protein in hemostasis.
- Accurate measurement of plasma fibrinogen is essential for diagnosing and managing bleeding disorders.
- Existing fibrinogen assays may be subject to various interferences.
Purpose of the Study:
- To present and evaluate three distinct methods for quantifying plasma fibrinogen.
- To assess the correlation and precision of these methods.
- To briefly discuss potential interferences affecting assay results.
Main Methods:
- Clottable protein assay utilizing Biuret reagent.
- Measurement of clot absorbance.
- Thrombin time assay.
Main Results:
- The three presented methods exhibit good correlation with each other.
- Adequate precision was observed for all methods in both normal and heparinized plasma samples.
- The study considered potential interferences from common substances.
Conclusions:
- The evaluated methods provide reliable quantification of plasma fibrinogen.
- These assays demonstrate robustness in the presence of heparin.
- Understanding potential interferences is crucial for accurate fibrinogen determination.