Detection of fumonisin B1: comparison of flow-injection liposome immunoanalysis with high-performance liquid...

Fumonisins are secondary metabolites of the fungus Fusarium moniliforme, a common mycotoxin in corn, which are known to cause cancer in a number of experimental animals and have been linked to human esophageal cancer in China and South Africa. A high-performance liquid chromatographic (HPLC) method is currently the most widely used method for the quantitative determination of fumonisins. This method utilizes precolumn derivatization with o-phthaldialdehyde, isocratic elution, and fluorescence detection. In this study, the HPLC method was chosen as the reference method to evaluate the reproducibility and accuracy of FILIA (flow-injection liposome immunoanalysis) for the detection of the fumonisin B1 (FmB1). Studies indicate that a recovery of 86-90% could be obtained when commercial yellow cornmeal spiked with FmB1 was extracted in 75% methanol, which correlated favorably (correlation coefficient, r(2)=0.945) with the result of 80-92% obtained using the flow-injection liposome immunoanalysis (FILIA) system. The data suggest that the FILIA method is comparable to HPLC for the detection of fumonisins in corn, animal feeds, and human foods. Important features of FILIA as compared to HPLC are, most importantly, lower detection limit (ca. 25 x lower), and also less complex and faster sample preparation and therefore increased analytical throughput. In addition, 24 human corn-based foods and 6 animal feeds were examined for the presence of FmB1 using HPLC and FILIA.