Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasmacytoid dendritic cells: the key to CpG.

Simon Rothenfusser1, Evelyn Tuma, Stefan Endres

  • 1Department of Internal Medicine, Division of Clinical Pharmacology, University of Munich, Munich, Germany.

Human Immunology
|December 14, 2002
PubMed
Summary

Plasmacytoid dendritic cells (PDCs) use toll-like receptor 9 (TLR9) to detect microbial DNA. PDCs can produce key immune-signaling molecules, influencing T helper cell responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RIG-I-targeted immunotherapy synergizes with immune checkpoint inhibition in a hepatocellular carcinoma model.

Molecular cancer therapeutics·2026
Same author

Correction: Serum CCL1 discriminates infectious and sterile systemic inflammation in sepsis and acute pancreatitis.

Scientific reports·2026
Same author

Dose Recommendations for Drugs in Patients With Liver Cirrhosis (The ALIVe Study): Protocol for a Multiphase Validation and Consensus Study.

JMIR research protocols·2026
Same author

5'-Triphosphate guanosine RNAs recruit GTP-binding proteins to suppress RIG-I/IFN type I signaling.

Molecular cell·2026
Same author

Serum CCL1 discriminates infectious and sterile systemic inflammation in sepsis and acute pancreatitis.

Scientific reports·2026
Same author

RIG-I Stimulation Enhances the Effector Function and Proliferation of Primary Human CD8+ T Cells.

International journal of molecular sciences·2026

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Vertebrate immune systems utilize toll-like receptor 9 (TLR9) to identify microbial DNA via CpG motifs.
  • In humans, TLR9 expression is limited to B cells and plasmacytoid dendritic cells (PDCs).

Purpose of the Study:

  • To investigate the role of PDCs in immune response initiation.
  • To understand how PDCs, expressing TLR7 and TLR9, recognize CpG DNA and influence T helper cell differentiation.

Main Methods:

  • Analysis of TLR expression profiles in dendritic cell subsets.
  • Assessment of cytokine production (IFN-alpha, IL-12) by PDCs upon stimulation with CpG DNA.
  • Evaluation of the impact of CD40 ligation and PDC differentiation stage on cytokine output.

Main Results:

Related Experiment Videos

  • PDCs exclusively express TLR7 and TLR9, with CpG DNA being the sole identified microbial ligand.
  • PDCs exhibit a unique capacity to produce high levels of both IFN-alpha and IL-12.
  • Cytokine production ratios are modulated by T helper cell costimulation (CD40 ligation), PDC differentiation, and CpG oligodeoxynucleotide (ODN) type.

Conclusions:

  • PDCs play a crucial role in establishing Th1 immune responses through CpG DNA recognition.
  • Under specific stimulation conditions, PDCs can promote Th1 responses, contrasting with their potential to support Th2/Th0 responses in the absence of CpG DNA.