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Cystite formation of Arthrobacter ureafaciens NRIC 0157(T)
Naoto Tanaka1, Tai Uchimura, Kazuo Komagata
1Laboratory of General and Applied Microbiology, Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan.
Abstract:
The cystite formation of Arthrobacter ureafaciens NRIC 0157(T) was studied by the use of a newly designed CT medium composed of 2.0% D-glucose, 0.28% NH(4)H(2)PO(4), 0.136% K(2)HPO(4) 0.136% KH(2)PO(4), 0.0005% MgSO(4).7H(2)O, and 0.0007% CaCl(2).2H(2)O. Cystites are drumstick-shaped and oval cells and are larger than vegetative cells. Cystites are Gram-negative, whereas vegetative cells are Gram-positive by the KOH reaction. The concentration and ratio of K(+) and Mg(2+) in CT medium mainly affected the cystite formation. Cystites are considered aberrant forms produced by nutritional imbalance.