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Updated: Aug 19, 2026
Experimental Metastasis Assay
Published on: August 25, 2010
[Differentially expressed gene in nasopharyngeal carcinoma cell lines with various metastatic potentialities]
Linjie Zhang1, Libing Song, Yinghong Ma
1Cancer Center, Sun Yat-sen University, Guangzhou 510060, China.
Objective:
To investigate gene expression profile in nasopharyngeaL carcinoma (NPC) cell lines with different metastatic potentialities, in order to identify new candidate genes related to the development, progress and metastasis of NPC.
Methods:
The mRNA expressions of high metastatic NPC cell line 5-8F, tumorigenic but nonmetastatic NPC cell line 6-10B and non-tumorigenic NPC cell line 13-9B (3 sublines of SUNE-1) were investigated by cDNA microarray containing 14 000 cDNA clones. The alterations in gene expression levels were confirmed by reverse-transcription PCR.
Results:
There were 82 differentially expressed genes comparing 5-8F and 13-9B; 38 differentially expressed genes comparing 6-10B and 13-9B; 54 comparing 5-8F and 6-10B. There were 12 common differentially expressed genes comparing 6-10B, 5-8F and 13-9B; 14 common differentially expressed genes comparing 5-8F and 13-9B, 6-10B. The expressions of the above genes were involved in metabolism, transcription, differentiation, apoptosis and signal transduction.
Conclusion:
The gene expression profile in nasopharyngeal carcinoma cell lines is an important index in the search of new candidate genes related to NPC.
