Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recent progress in derivatization methods for LC and CE analysis.

Takeshi Fukushima1, Noriko Usui, Tomofumi Santa

  • 1Laboratory of Bio-Analytical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Journal of Pharmaceutical and Biomedical Analysis
|December 18, 2002
PubMed
Summary

Derivatization enhances detection sensitivity and selectivity in chromatography. This technique achieves femtomol detection limits in HPLC and attomole levels in CE for biosample analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epidemiology of musculoskeletal injuries in Chinese collegiate dragon boat athletes: a cross-sectional survey.

Frontiers in public health·2026
Same author

Rapid immunohistochemistry for intraoperative differentiation between high-grade gliomas and primary central nervous system lymphomas: a multicenter prospective study.

Brain tumor pathology·2026
Same author

The Characteristics of Training Injuries Among Chinese Competitive Rugby Sevens Players: A Retrospective Study.

Healthcare (Basel, Switzerland)·2026
Same author

Oxidized Albumin: An Emerging Biomarker Bridging Oxidative Stress and Renal Pathophysiology.

Biomedical chromatography : BMC·2026
Same author

Epidemiological Characteristics of Pain Among Rowers: A Retrospective Questionnaire Study.

Journal of pain research·2026
Same author

The Epidemiology of Premenstrual Syndrome and Sports Injuries Among National-Level Adolescent Female Badminton Athletes.

Healthcare (Basel, Switzerland)·2026

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Separation Science

Background:

  • Chromatographic techniques like HPLC and CE are crucial for biosample analysis.
  • Enhancing detection sensitivity and selectivity is vital for accurate quantification.
  • Derivatization is a key strategy to improve analyte detectability.

Purpose of the Study:

  • To review recent derivatization methods for HPLC and CE.
  • To highlight the application of these methods in biosample analysis.
  • To demonstrate the improvement in detection limits and analysis time.

Main Methods:

  • Utilizing fluorescence and chemiluminescence reagents for derivatization.
  • Applying derivatization in High-Performance Liquid Chromatography (HPLC).

Related Experiment Videos

  • Implementing derivatization in Capillary Electrophoresis (CE) with laser-induced fluorescence or near infra-red detection.

    • Main Results:

    • Achieved femtomol order mass detection limits in HPLC via derivatization.
    • Demonstrated attomole to yoctomole level detection limits in CE.
    • Reported very short analysis times (under a few minutes) in CE.
    • Highlighted the effectiveness of water-soluble fluorescence reagents for peptide determination, preventing adsorption.

    Conclusions:

    • Derivatization significantly boosts detection sensitivity and selectivity in HPLC and CE.
    • Advanced detection techniques like laser-induced fluorescence in CE enable ultra-trace analysis.
    • Derivatization is essential for sensitive and selective analysis of complex biosamples.