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Sensor and biosensor based on Prussian Blue modified gold and platinum screen printed electrodes.
Ivanildo Luiz de Mattos1, Lo Gorton, Tautgirdas Ruzgas
1Department of Analytical Chemistry, University of Lund, P.O. Box 124, S-22100 Lund, Sweden.
Biosensors & Bioelectronics
|December 18, 2002
Summary
Screen-printed electrodes modified with Prussian Blue enable selective detection of hydrogen peroxide and glucose. These amperometric sensors demonstrate high sensitivity and improved operational stability for reliable long-term monitoring.
Area of Science:
- Electroanalytical Chemistry
- Biosensor Development
- Materials Science
Background:
- Screen-printed electrodes (SPEs) offer a versatile platform for electrochemical sensing.
- Prussian Blue (PB) is a redox-active material suitable for modifying electrodes.
- Hydrogen peroxide and glucose are important analytes in various fields, necessitating accurate detection methods.
Purpose of the Study:
- To develop selective amperometric sensors for hydrogen peroxide detection using Prussian Blue-modified gold (Au) and platinum (Pt) screen-printed electrodes.
- To construct glucose biosensors based on these modified electrodes by immobilizing glucose oxidase.
- To evaluate the operational stability and long-term performance of the developed sensors and biosensors.
Main Methods:
- Modification of Au and Pt screen-printed electrodes with Prussian Blue.
- Immobilization of crystallized glucose oxidase in a Nafion membrane for glucose biosensor fabrication.
- Use of a buffer solution containing tetrabutylammonium toluene-4-sulfonate to enhance operational stability.
- Evaluation of sensor performance using flow-injection analysis for continuous monitoring.
Main Results:
- The developed sensors showed high sensitivity towards hydrogen peroxide: 2 A M⁻¹ cm⁻² for Au SPEs and 1 A M⁻¹ cm⁻² for Pt SPEs.
- The Prussian Blue-modified electrodes were successfully utilized for the construction of glucose biosensors.
- Improved operational stability was achieved using the specified buffer solution.
- Continuous monitoring for 10 hours demonstrated the long-term performance of the sensors and biosensors.
Conclusions:
- Prussian Blue-modified screen-printed electrodes are effective for selective hydrogen peroxide detection.
- These platforms can be readily adapted for glucose biosensing applications.
- The use of specific additives enhances the stability and durability of electrochemical sensors for practical applications.