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Updated: Sep 28, 2026

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
Published on: June 24, 2018
Possible role of extracellular matrix vesicles in initial calcification of healing rachitic cartilage
Abstract:
Fluid (20-30 nl) was aspirated by a modified renal micropuncture technique from vitamin D-, phosphate-deficient rats. The fluid revealed a mineral forming agent which could be sedimented at 140,000 X g for 8 hours, was resistant to acid demineralization, but was destroyed by heating, freezing and thawing as well as sonication, and blocked by phospholipase A at 10-5 M but not at 10-7 M. Electron microscopic study of the fluid sediment revealed images consistent with matrix vesicles. These data are consonant with the view that matrix vesicles, their remnants, or closely associated structures comprised the mineral forming agent.
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