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Co-eluent effect in partition chromatography. Rhamnose-xylose separation with strong and weak cation-exchangers in
Jari Tiihonen1, Tuomo Sainio, Ari Kärki
1Lappeenranta University of Technology, Laboratory of Industrial Chemistry, P.O. Box 20, FIN-53851 Lappeenranta, Finland. jari.tiihonen@lut.fi
Abstract:
The effect of ethanol in aqueous eluent on the chromatographic separation was studied at 298 K. Two sugars, L-rhamnose and D-xylose, were separated by using strong and weak cation-exchangers as a stationary phase. The ionic form of the resins was Na+ or Ca2+. The separations were carried out with sugar feed concentrations up to 35 wt% and with both low (about 1%) and high (about 10%) feed volume to bed volume ratios. The separation of the sugars was improved by adding ethanol into the eluent. The separation was also significantly enhanced when the weak cation-exchangers with the greatest affinity for water were used instead of strong cation-exchangers as a separation medium for the sugars having different hydrophilicities. The experimental data were successfully explained with a rate-based column model, which accounted for the volume changes of the stationary phase. A thermodynamic sorption model was utilized in column calculations.
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