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Related Experiment Videos

Herpesvirus papio 2 encodes a virion host shutoff function.

John E Bigger1, David W Martin

  • 1Department of Virology and Immunology, Southwest Foundation for Biomedical Research, Texas 78227-5301, USA.

Virology
|December 20, 2002
PubMed
Summary
This summary is machine-generated.

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Herpesvirus papio 2 (HVP-2) in baboons mimics human herpes simplex virus (HSV) infections. A key viral gene, vhs, was identified, which shuts down host cell protein synthesis and degrades mRNA.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Herpesvirus papio 2 (HVP-2) infection in baboons causes disease analogous to human herpes simplex virus (HSV) infections.
  • HVP-2 virions possess a factor that rapidly inhibits host cell protein synthesis post-infection.
  • This inhibition correlates with the degradation of cellular messenger RNA (mRNA) species.

Purpose of the Study:

  • To molecularly characterize the HVP-2 virion host shutoff (vhs) gene homolog.
  • To investigate the role of the HVP-2 vhs gene in inhibiting host protein synthesis and degrading mRNA.
  • To establish the HVP-2 baboon model for studying vhs function and developing vhs-deficient virus vaccines.

Main Methods:

  • Southern blot and DNA sequence analysis to identify the HVP-2 vhs gene homolog.

Related Experiment Videos

  • Comparison of the HVP-2 vhs gene sequence with those of HSV-1 and HSV-2.
  • Disruption of the HVP-2 vhs open reading frame to assess its function.

    • Main Results:

    • A HVP-2 gene with significant sequence identity (>70%) to HSV vhs genes was identified.
    • Disruption of the HVP-2 vhs gene significantly reduced the virus's ability to shut off protein synthesis.
    • The disruption also diminished the degradation of cellular mRNA, confirming the gene's role in vhs activity.

    Conclusions:

    • The HVP-2 vhs gene homolog is responsible for the virion host shutoff activity observed in HVP-2 infections.
    • The HVP-2 baboon model offers a valuable system for studying the biological significance of vhs in a natural primate host.
    • This model can be utilized for preclinical testing of vaccines based on vhs-deficient viruses.