Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Freezing effect on sperm DNA].

Bo Song1, Lü-Kang Zheng, Li-Xia Deng

  • 1Department of Genetic Research, Sun Yat-San University of Medical Science, Guangzhou, Guangdong 510089, China. songbo58@163.com

Zhonghua Nan Ke Xue = National Journal of Andrology
|December 21, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unraveling the Raman Enhancement Mechanism on 1T'-Phase ReS<sub>2</sub> Nanosheets.

Small (Weinheim an der Bergstrasse, Germany)·2018
Same author

Impact of overexpression of cytosolic isoform of O-acetylserine sulfhydrylase on soybean nodulation and nodule metabolome.

Scientific reports·2018
Same author

Heavy metal contents and enrichment characteristics of dominant plants in wasteland of the downstream of a lead-zinc mining area in Guangxi, Southwest China.

Ecotoxicology and environmental safety·2018
Same author

Quantitative Monitoring and Visualization of Hydrogen Sulfide In Vivo Using a Luminescent Probe Based on a Ruthenium(II) Complex.

Angewandte Chemie (International ed. in English)·2018
Same author

Accurate virial coefficients of gaseous krypton from state-of-the-art ab initio potential and polarizability of the krypton dimer.

The Journal of chemical physics·2018
Same author

Reversible colour change in leaves enhances pollinator attraction and reproductive success in Saururus chinensis (Saururaceae).

Annals of botany·2018
Same journal

[Research progress on multimodal precision nursing interventions in urinary incontinence rehabilitation management after radical prostatectomy].

Zhonghua nan ke xue = National journal of andrology·2025
Same journal

[Dietary intervention to improve erectile dysfunction: Progress in research].

Zhonghua nan ke xue = National journal of andrology·2025
Same journal

[Tonifying kidney and activating blood therapy for the treatment of diabetic erectile dysfunction: A systematic review and meta-analysis].

Zhonghua nan ke xue = National journal of andrology·2025
Same journal

[Clinical effect of Huotujiji prescription on patients with asthenospermia of spleen-kidney yang-deficiency type].

Zhonghua nan ke xue = National journal of andrology·2025
Same journal

[Application of predictive nursing to the rehabilitation of patients after endoscopic surgery for prostate under the concept of enhanced recovery after surgery].

Zhonghua nan ke xue = National journal of andrology·2025
Same journal

[Influencing factors of lower urinary tract symptoms in patients after radical prostatectomy and nursing strategy].

Zhonghua nan ke xue = National journal of andrology·2025
See all related articles

Freezing sperm at -80°C does not harm sperm DNA integrity. Studies using single cell gel electrophoresis (SCGE) found no statistical difference over time, confirming DNA stability in frozen states.

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Cryobiology

Context:

  • Sperm preservation is crucial for assisted reproductive technologies.
  • Understanding the impact of cryopreservation on sperm DNA is essential for successful outcomes.
  • Previous studies have yielded conflicting results regarding DNA damage after freezing.

Purpose:

  • To evaluate the effect of cryopreservation at -80°C on sperm DNA integrity.
  • To determine if freezing temperatures compromise sperm DNA stability over time.

Summary:

  • Sperm DNA was assessed using single cell gel electrophoresis (SCGE) after preservation at -80°C.
  • Analysis of variance (ANOVA) was employed to analyze the time factor.
  • No statistically significant difference in sperm DNA integrity was observed over the study period.

Related Experiment Videos

Impact:

  • Cryopreservation at -80°C appears to maintain sperm DNA integrity.
  • Findings support the reliability of sperm banking for future use.
  • This research contributes to optimizing sperm preservation protocols in fertility treatments.