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Urinary free light chain analysis by the Freelite immunoassay: a preliminary study in multiple myeloma
Thierry Le Bricon1, Djaouida Bengoufa, Mourad Benlakehal
1Laboratoire de Biochimie A, Hôpital St-Louis AP-HP, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France. thierry.le-bricon@sls.ap-hop-paris.fr
Clinical Biochemistry
|December 21, 2002
Abstract:
Evaluate the Freelite free light chain immunoassay for urine analysis in myeloma. Urine specimens from 20 patients were analyzed by Freelite (The Binding Site) and SDS-agarose gel electrophoresis (Hydragel protéinurie, Sebia). Using the kappa/lambda ratio, Freelite was more sensitive than electrophoresis to detect free light chains, but concentration was overestimated in 75% of cases. Despite high sensitivity and full automation, Freelite inaccurately measures monoclonal free light chains in urine.