Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Embryo technologies in the horse.

E L Squires1, E M Carnevale, P M McCue

  • 1Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO 80523, USA. esquires@cvmbs.colostate.edu

Theriogenology
|December 25, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of cost for conventional embryo transfer versus intracytoplasmic sperm injection.

Journal of equine veterinary science·2026
Same author

Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares.

Frontiers in cell and developmental biology·2024
Same author

Equine maternal aging affects the metabolomic profile of oocytes and follicular cells during different maturation time points.

Frontiers in cell and developmental biology·2023
Same author

Alterations of Circulating Biomarkers During Late Term Pregnancy Complications in the Horse Part I: Cytokines.

Journal of equine veterinary science·2021
Same author

Changes in circulating concentrations of testosterone and estrone sulfate after human chorionic gonadotropin administration and subsequent to castration of 2-year-old stallions.

Animal reproduction science·2020
Same author

Equine endometritis: a review of challenges and new approaches.

Reproduction (Cambridge, England)·2020
Same journal

Possible actions of methionine on embryonic development and postnatal phenotype of calves produced after embryo transfer.

Theriogenology·2026
Same journal

Telomere regulation of mitochondria in porcine parthenogenetic embryos.

Theriogenology·2026
Same journal

Interaction of ARA54 with androgen receptor in mediating testosterone-dependent regulation of caput epididymal GPX5 expression in mice.

Theriogenology·2026
Same journal

Associations of sow characteristics, boar semen traits, and seminal plasma metabolomics with fertility outcomes following artificial insemination.

Theriogenology·2026
Same journal

Association of 17β-HSD3 with steroidogenesis-related gene expression and primordial germ cell development in ducks.

Theriogenology·2026
Same journal

Farrerol improves the maturation quality of porcine oocytes derived from small follicles associated with RAD51-related DNA repair responses.

Theriogenology·2026
See all related articles

Zwitterionic buffers enable satisfactory equine embryo storage at 5°C. Assisted reproductive techniques improve pregnancy rates in older mares and advance equine breeding technologies.

Area of Science:

  • Reproductive biology
  • Embryology
  • Veterinary science

Background:

  • Equine embryo cryopreservation and assisted reproductive techniques (ART) are crucial for managing subfertile mares and advancing equine breeding.
  • Traditional methods face challenges with equine oocyte cryopreservation and in vitro embryo production.
  • Superovulation in horses is limited, necessitating methods to maximize embryo recovery.

Purpose of the Study:

  • To review recent advancements in equine embryo storage, assisted reproduction, and cryopreservation techniques.
  • To highlight the efficacy of ART in improving pregnancy rates for older or subfertile mares.
  • To discuss the potential and current limitations of in vitro embryo production and oocyte cryopreservation in horses.

Main Methods:

  • Review of recent studies on zwitterionic buffer efficacy for equine embryo storage.

Related Experiment Videos

  • Analysis of data on pregnancy rates using in vivo-matured oocyte transfer in mares of varying fertility.
  • Examination of intracytoplasmic sperm injection (ICSI) and gamete intrafallopian tube transfer (GIFT) outcomes.
  • Evaluation of equine oocyte vitrification success rates using cryoloop technology.

    • Main Results:

    • Zwitterionic buffers provide satisfactory equine embryo storage at 5°C.
    • Transfer of in vivo-matured oocytes yields higher pregnancy rates (70-80%) from young mares compared to older mares (30-40%).
    • Intracytoplasmic sperm injection (ICSI) has resulted in foal production with reported cleavage and blastocyst rates.
    • Equine oocyte vitrification has shown success, leading to live foal production.

    Conclusions:

    • Zwitterionic buffers offer a viable option for equine embryo storage.
    • Assisted reproductive techniques significantly enhance reproductive success in older and subfertile mares.
    • While challenges remain in equine in vitro embryo production, techniques like ICSI and oocyte vitrification show promise for the future of equine reproduction and cloning.