Related Experiment Video
Updated: Sep 28, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
[PCR amplification and cloning of virulence expression regulatory gene toxR of Vibrio cholerae]
1Department of Parasitology, School of Basic Medical Sciences, WCUMS, Chengdu 610041.
Abstract:
In order to construct a genomic bivalent oral vaccine of Leishmania donovani and Vibrio cholerae, we amplified a 1.3 kb DNA fragment from 7 strains of Vibrio cholerae with primers P1 and P2. Restriction endonuclease analysis of PCR amplified products from 9 strains of Vibrio cholerae was performed by digestion with EcoR I. The results revealed an EcoR I site in the central part of toxR gene. The entire toxR gene of Vibrio cholerae Non-CT strain 7743 was amplified by PCR with primers P1 and P2, digested with endonucleases BamH I and Hind III, then was orientationally cloned into plasmid pAT153. The restriction endonuclease analysis demonstrates that the recombinant plasmid ptR4 contains a 1.3 kb toxR gene fragment.
Related Concept Videos
Regulation of Bacterial Virulence
Cholera
Bacterial Toxins
Bacterial Transformation
Bacterial Transformation

