[The effect of heparin on mesangial cells proliferation and cytokines production]
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Abstract:
The human glomerular mesangial cells(MC) proliferation and cytokines secretion were detected by using MTT method and biological active method so as to assess the effects of heparin on the cultured human glomerular MC induced by lipopolysaccharides (LPS). The results showed that there were basic production of interleukin-6(IL-6) and tumor necrosis factor-alpha (TNF-alpha) on MC in the control group without LPS, that high-concentration heparin(500 U/ml) increased the effects of LPS on MC, and that low-concentration heparin (5 U/ml) conversely inhibited the effects of LPS on MC. These data indicate that the low-concentration heparin could inhibit the proliferation of MC and the secrection of IL-6 and TNF-alpha induced by LPS, and hence provide an experimental evidence for administration of heparin in the treatment glomerular disease.
Insights
Low-concentration heparin inhibits lipopolysaccharide-induced human glomerular mesangial cell proliferation and cytokine secretion. This finding offers experimental support for using heparin in treating glomerular diseases.
Area of Science:
- Nephrology
- Immunology
- Pharmacology
Background:
- Glomerular diseases involve mesangial cell (MC) proliferation and cytokine release.
- Lipopolysaccharides (LPS) can induce MC activation.
- Heparin's role in modulating these processes requires clarification.
Purpose of the Study:
- To investigate the effects of different heparin concentrations on LPS-induced human MC proliferation and cytokine secretion.
- To assess heparin's potential as a therapeutic agent for glomerular diseases.
Main Methods:
- Cultured human glomerular mesangial cells (MC) were treated with LPS.
- The MTT assay was used to measure cell proliferation.
- Biological activity assays detected cytokine secretion (IL-6, TNF-alpha).
- Cells were exposed to varying concentrations of heparin (low: 5 U/ml, high: 500 U/ml).
Main Results:
- Basal production of IL-6 and TNF-alpha was observed in MC without LPS.
- High-concentration heparin (500 U/ml) potentiated LPS-induced effects on MC.
- Low-concentration heparin (5 U/ml) significantly inhibited LPS-induced MC proliferation and IL-6/TNF-alpha secretion.
Conclusions:
- Low-concentration heparin demonstrates an inhibitory effect on LPS-stimulated human MC.
- Heparin reduces MC proliferation and pro-inflammatory cytokine release.
- These findings provide experimental evidence supporting heparin's use in managing glomerular diseases.
