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[Feasibility of calcium polyphosphate fiber as scaffold materials for tendon tissue engineering in vitro]
1Orthopaedic Institute of Lanzhou Medical College, Lanzhou Gansu, P. R. China 730030.
Objective:
To study the feasibility of calcium polyphosphate fiber (CPPF) as the scaffold material of tendon tissue engineering.
Methods:
CPPF (15 microns in diameter) were woven to form pigtail of 3 mm x 2 mm transverse area; and the tensile strength, porous ratio and permeability ratio were evaluated in vitro. Tendon cells (5 x 10(4)/ml) derived from phalangeal flexor tendon of SD rats were co-culture with CPPF scaffold or CPPF scaffold resurfaced with collagen type-I within 1 week. The co-cultured specimens were examined under optical and electric scanning microscope.
Results:
The tensile strength of CPPF scaffolds was (122.80 +/- 17.34) N; permeability ratio was 61.56% +/- 14.57%; and porous ratio was 50.29% +/- 8.16%. CPPF had no obvious adhesive interaction with tendon cells, while CPPF of surface modified with collagen type-I showed good adhesive interaction with tendon cells.
Conclusion:
The above results show that CPPF has some good physical characteristics as scaffold of tendon tissue engineering, but its surface should be modified with organic substance or even bioactive factors.