Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hyperinsulinemia and oxidative stress.

P Kyselová1, M Zourek, Z Rusavý

  • 1Department of Internal Medicine, University Hospital, Pilsen, Czech Republic.

Physiological Research
|January 4, 2003
PubMed
Summary

Short-term hyperinsulinemia, not hyperglycemia alone, reduced oxidative stress markers in rats. Exogenous insulin lowered reactive oxygen species (ROS) production during high blood sugar.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

False-Positive Cardiac Troponin Elevations in Skeletal Muscle Disease: Clinical Relevance, Mechanisms, and Laboratory Approaches.

Physiological research·2025
Same author

Effect of Beer Consumption on Methylation and Redox Metabolism.

Physiological research·2022
Same author

Age-Related Progression of Microvascular Dysfunction in Cystic Fibrosis: New Detection Ways and Clinical Outcomes.

Physiological research·2021
Same author

Levator ani avulsion: a Systematic evidence review (LASER).

BJOG : an international journal of obstetrics and gynaecology·2021
Same author

Vaginal birth after Cesarean section and levator ani avulsion: a case-control study.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2021
Same author

NGAL, albumin and cystatin C during cisplatin therapy.

Physiological research·2020

Area of Science:

  • Biochemistry
  • Physiology
  • Endocrinology

Background:

  • Oxidative stress plays a crucial role in metabolic disorders.
  • Understanding the interplay between glucose and insulin levels is vital for metabolic health.

Purpose of the Study:

  • To investigate the distinct effects of short-term hyperglycemia and hyperinsulinemia on oxidative stress parameters.
  • To compare oxidative stress markers under different glycemic and insulinemic conditions in Wistar rats.

Main Methods:

  • Hyperinsulinemic-euglycemic clamp technique was employed in Wistar rats.
  • Measurements included malondialdehyde (MDA), reduced glutathione (GSH), and total antioxidant capacity (AOC).
  • Rats were subjected to isolated hyperglycemia or combined hyperglycemia and hyperinsulinemia.

Main Results:

  • Total antioxidant capacity (AOC) remained stable across all conditions.
  • Malondialdehyde (MDA), a marker of lipid peroxidation, decreased with hyperinsulinemia but increased with isolated hyperglycemia.
  • Reduced glutathione (GSH) levels decreased significantly during isolated hyperglycemia.

Conclusions:

  • Short-term exogenous hyperinsulinemia mitigates the increase in reactive oxygen species (ROS) production associated with hyperglycemia.
  • Insulin, independent of glucose levels, appears to have a protective effect against oxidative stress.
  • These findings highlight the potential therapeutic role of insulin in managing hyperglycemia-induced oxidative damage.

Related Experiment Videos