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Proviral DNA of Moloney leukemia virus: purification and visualization

A M Gianni, J R Hutton, D Smotkin

Science (New York, N.Y.)

|February 13, 1976

Abstract:

Closed-circular proviral DNA of Moloney leukemia virus has been purified from a 10(7) excess of cellular and mitochondrial DNA. The DNA can be visualized in the electron microscope and has the contour length of a molecule with a moleculecular weight of about 5.5 + 10(6). Electron microscopic observation of a hybrid between viral RNA and this circular DNA confirms the viral origin of this molecule.

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