Related Experiment Video
Updated: Sep 27, 2026
HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Determination of furostanol saponins in Bulbus Allii Macroste]
1Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110015, Liaolin, China.
Objective:
To establish an Ehrlich reaction-colorimetry method for determining furostanol saponins in Bulbus Allii Macroste.
Method:
Based on the coloration reaction between furostanol saponins and p-dimethylaminobenzaldehyde, the UV detection wavelength used was 515 nm, and the optimum conditions were selected on homogeneous design principle.
Result:
The standard curve of macrostemonoside G was linear in the concentration range of 0.1975-0.9880 mg.ml-1. The average recovery was 97.7% (RSD = 3.65%).
Conclusion:
The method is rapid, accurate and easy to operate.