Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydrogen peroxide modulation of synaptic plasticity.

Ariel Kamsler1, Menahem Segal

  • 1Department of Neurobiology, The Weizmann Institute, Rehovot 76100, Israel.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|January 7, 2003
PubMed
Summary

Hydrogen peroxide (H2O2) impacts synaptic plasticity differently based on concentration. Low H2O2 enhances long-term potentiation (LTP), while high concentrations inhibit it, suggesting complex roles in brain function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synaptic potentiation requires PARP1 activation: prevailing concepts are revisited.

Molecular psychiatry·2026
Same author

Calcium signaling in postsynaptic mitochondria: mechanisms, dynamics, and role in ATP production.

Frontiers in molecular neuroscience·2025
Same author

Distinct Ventral Hippocampus Network Properties in Dissociated Cultures.

Hippocampus·2024
Same author

Cultured Rat Hippocampal Neurons Exposed to the Mitochondrial Uncoupler Carbonyl Cyanide Chlorophenylhydrazone Undergo a Rapid, Presenilin-Dependent Change in Neuronal Properties.

International journal of molecular sciences·2024
Same author

Exposure of Cultured Hippocampal Neurons to the Mitochondrial Uncoupler Carbonyl Cyanide Chlorophenylhydrazone Induces a Rapid Growth of Dendritic Processes.

International journal of molecular sciences·2023
Same author

Dynamic Regulation of Mitochondrial [Ca<sup>2+</sup>] in Hippocampal Neurons.

International journal of molecular sciences·2022

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Biochemistry

Background:

  • Reactive oxygen species (ROS), including hydrogen peroxide (H2O2), are implicated in neurodegeneration.
  • The acute effects of ROS on synaptic plasticity remain largely unexplored.

Purpose of the Study:

  • To investigate the concentration-dependent effects of H2O2 on synaptic plasticity in rat hippocampal slices.
  • To elucidate the molecular mechanisms underlying H2O2-mediated alterations in long-term potentiation (LTP) and long-term depression (LTD).

Main Methods:

  • Electrophysiological recordings of excitatory postsynaptic potentials (EPSPs) in rat hippocampal slices.
  • Application of varying concentrations of H2O2 (1 µM to 5 mM).
  • Pharmacological manipulation using L-type calcium channel blockers (nifedipine) and calcineurin inhibitors (FK506, cyclosporin A).

Related Experiment Videos

Main Results:

  • High H2O2 concentrations (0.5-5 mM) reduced EPSPs.
  • 20 µM H2O2 inhibited new LTP induction and enhanced LTD.
  • 1 µM H2O2 significantly increased LTP, particularly NMDA-independent LTP, and suppressed LTD.
  • Nifedipine blocked H2O2 effects, while calcineurin inhibitors restored LTP in the presence of 20 µM H2O2.

Conclusions:

  • H2O2 exhibits biphasic effects on synaptic plasticity, with low concentrations enhancing LTP and high concentrations impairing it.
  • H2O2-induced alterations in NMDA-independent LTP involve voltage-gated calcium channels and calcineurin activation.
  • These findings reveal a novel role for H2O2 in modulating synaptic plasticity, distinct from its proposed role in neurodegeneration.