[Sequence analysis of Ad7 vaccine strain 87 mu-97.4 mu fragment]

Adenovirus 7 vaccine strain(Ad7v) possesses potentiality to be used as a vector for producing recombinant vaccine. In order to construct Ad7 vector, the nucleotide sequence of a 3698 bp fragment(87 to 97.4 mu) of the virus DNA was analysed. The fragment encodes three ORFs namely, one ORF of early region 4(E4), one ORF of early region 3 and one ORF of fiber protein. The predicted amino acids of three ORFs(ORF 8.1 kD, 42.8 kD and 10.3 kD) share 33%, 43% and 44% homology with Ad2 E4 corresponding regions (ORF 3, ORF 4 and ORF 6/7) respectively. A comparison of the nucleotide and amino acid sequences of those fiber ORF demonstrated a higher homology between Ad7v and prototype strains of Ad7v than those between Ad7v and Ad7h. The Ad7v E3 15.4 kD protein shares 86%, 88%, 90% and 98.5% amino acid homology with the equivalent region of ad7h E3 15.3 kD protein, Ad4 E3 region 15 kD protein, Ad3 E3 15.3 kD and Ad7p E3 15.3 kD protein protein respectively, but only 68% with Ad2 E3 14.7 kD protein.