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[Comparison of infectious brain edema models induced by different kinds of pertussis bacilli in rats]
1Department of Pediatrics, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Objective:
To understand the difference of infectious brain edema models induced by varied kinds of pertussis bacilli in rats and to look for a new infectious brain edema model.
Methods:
Sprague-Dawley rats were randomly divided into 3 groups: normal saline group(NS); rude pertussis bacilli(PBR); purified pertussis bacilli(PBP). After infectious brain edema model was induced by rude pertussis bacilli or purified pertussis bacilli, the water content(WC), Evan's blue content(EB), Na+ and K+ content in brain tissues were measured.
Results:
The WC, EB and Na+ contents in brain tissues in PBR and PBP groups were significantly increased than those in NS group; K+ content was significantly decreased in PBR and PBP groups than that in NS group(P < 0.01). Evan's blue discoloration was demonstrated in all rats of PBR group, but 6 rats of PBP group showed bluish discoloration. EB content in brain tissues was significantly increased in PBR group than that in PBP group.
Conclusions:
The infectious brain edema model can be induced by rude pertussis bacilli or purified pertussis bacilli in rats, the former gives priority to mixed brain edema, and the latter mainly avails cytotoxin brain edema.
Insights
Researchers developed infectious brain edema models in rats using both rude and purified pertussis bacilli. Rude pertussis bacilli induced mixed brain edema, while purified pertussis bacilli primarily caused cytotoxin brain edema.
Area of Science:
- Neurology
- Infectious Diseases
- Toxicology
Background:
- Infectious brain edema poses a significant health challenge.
- Understanding the specific mechanisms of edema induction is crucial for developing effective treatments.
- Pertussis bacilli are known pathogens that can affect the central nervous system.
Purpose of the Study:
- To differentiate the effects of rude versus purified pertussis bacilli on infectious brain edema in a rat model.
- To establish a reliable and reproducible model for studying infectious brain edema.
Main Methods:
- Sprague-Dawley rats were divided into three groups: normal saline, rude pertussis bacilli (PBR), and purified pertussis bacilli (PBP).
- Infectious brain edema models were induced using PBR or PBP.
- Brain tissue water content (WC), Evan's blue (EB) content, and Na+/K+ levels were measured.
Main Results:
- Both PBR and PBP groups showed significantly increased brain tissue WC, EB, and Na+ content compared to the normal saline group.
- K+ content was significantly decreased in both PBR and PBP groups.
- Evan's blue discoloration was observed in all PBR rats, but only in 6 PBP rats, with higher EB content in the PBR group.
Conclusions:
- Both rude and purified pertussis bacilli can induce infectious brain edema models in rats.
- Rude pertussis bacilli predominantly induce mixed brain edema.
- Purified pertussis bacilli primarily induce cytotoxin-mediated brain edema.
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