Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cellular genomics: which genes are transcribed, when and where?

Ana Pombo1

  • 1MRC - Clinical Sciences Centre, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Hammersmith Hospital Campus, Du Cane Road, W12 0NN, London, UK. ana.pombo@csc.mrc.ac.uk

Trends in Biochemical Sciences
|January 9, 2003
PubMed
Summary

Cellular genomics uses single-cell imaging to analyze gene expression. This approach links mRNA levels to the underlying transcriptional events within individual cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Haplotype-resolved genome architecture mapping uncovers pervasive structural heterogeneity between human homologous chromosomes.

bioRxiv : the preprint server for biology·2026
Same author

An integrated view of the structure and function of the human 4D nucleome.

Nature·2025
Same author

Specialised super-enhancer networks in stem cells and neurons.

bioRxiv : the preprint server for biology·2025
Same author

Cryosectioning-enhanced super-resolution microscopy for single-protein imaging across cells and tissues.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Extensive folding variability between homologous chromosomes in mammalian cells.

Molecular systems biology·2025
Same author

Oncogene Silencing via ecDNA Micronucleation.

bioRxiv : the preprint server for biology·2025

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Gene expression is a complex process involving multiple molecular events.
  • Messenger RNA (mRNA) profiling provides insights into total RNA content but doesn't directly correlate with transcription.
  • Understanding the link between mRNA levels and active transcription in single cells is crucial.

Purpose of the Study:

  • To explore the potential of single-cell imaging for analyzing transcriptional activity.
  • To bridge the gap between mRNA profiling and the dynamic events of gene expression.

Main Methods:

  • Utilizing recent advances in single-cell imaging techniques.
  • Implementing high-throughput analysis for 'cellular genomics'.

Main Results:

Related Experiment Videos

  • Single-cell imaging enables direct observation of transcriptional activity.
  • This method allows for the analysis of gene expression at the individual cell level.

Conclusions:

  • Cellular genomics, powered by single-cell imaging, offers a novel platform for studying gene expression.
  • This approach provides a more detailed understanding of transcriptional regulation and mRNA dynamics in single cells.