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Platelet activation and PDGF-AB release during dialysis
G Donati1, G Cianciolo, F D'Addio
1Nephrology Dialysis and Renal Transplantation Unit, Department of Clinical Medicine and Applied Biotechnology, S. Orsola University Hospital, Bologna, Italy. emodia@orsola-malpighi.med.unibo.it
The International Journal of Artificial Organs
|January 10, 2003
Summary
Hemodialysis triggers the release of Platelet Derived Growth Factor (PDGF-AB) from blood-membrane contact. Levels remain elevated post-dialysis, potentially contributing to atherosclerosis risk in patients.
Area of Science:
- Nephrology
- Cardiovascular Research
- Biochemistry
Background:
- Blood-membrane interaction during hemodialysis (HD) causes platelet activation.
- Platelet Derived Growth Factor (PDGF-AB) release is implicated in accelerated atherosclerosis.
- Understanding PDGF-AB kinetics during HD is crucial for patient risk assessment.
Purpose of the Study:
- To evaluate intra- and post-dialytic changes in serum PDGF-AB levels.
- To compare PDGF-AB release using Hemophan (HE) and Polysulfone (PS) dialysis membranes.
- To investigate the correlation between PDGF-AB and other platelet activation markers.
Main Methods:
- Serum levels of PDGF-AB, PF4, betaTG, and MPV were measured in 30 HD patients.
- Patients underwent 3 HE and 3 PS membrane dialysis sessions.
- Blood samples collected during and up to 20 hours post-dialysis.
Main Results:
- PDGF-AB levels increased during HD, remaining high post-session for up to 20 hours.
- PF4, betaTG, and MPV exhibited similar trends to PDGF-AB.
- No significant difference in PDGF-AB release was observed between HE and PS membranes.
Conclusions:
- Blood-membrane contact during HD consistently releases PDGF-AB, irrespective of membrane type.
- Elevated PDGF-AB levels persist long after dialysis, suggesting a potential role in atherosclerosis progression.
- PDGF-AB release during HD may act as an additional risk factor for atherosclerotic lesions in dialysis patients.