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Updated: Jul 30, 2026
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Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Published on: November 8, 2011
[MTHFR gene expression in EB virus transformed human B lymphoblasts]
Wei Sheng Yan Jiu = Journal of Hygiene Research
|January 11, 2003
Summary
The N5, N10-methylene-tetrahydrofolate reductase (MTHFR) gene is expressed in Epstein-Barr virus (EBV)-transformed human B lymphoblasts. Its cDNA sequence closely matches the human liver MTHFR gene sequence.
Area of Science:
- Molecular Biology
- Genetics
- Virology
Context:
- Epstein-Barr virus (EBV) is known to transform human B lymphocytes.
- Understanding gene expression in transformed cells is crucial for cellular and molecular biology research.
Purpose:
- To detect the expression of the N5, N10-methylene-tetrahydrofolate reductase (MTHFR) gene in EBV-transformed human B lymphoblasts.
- To determine the cDNA sequence of the MTHFR gene in this cell line.
Summary:
- Human peripheral blood was used to create an EBV-transformed B lymphoblast cell line.
- Total RNA was isolated, and MTHFR cDNA segments were amplified using RT-PCR and analyzed by PAGE.
- Sequencing of a cDNA segment confirmed MTHFR gene expression and revealed a high similarity to human liver MTHFR cDNA.
Impact:
- This study confirms MTHFR gene expression in a relevant cellular model for EBV research.
- The findings provide a molecular basis for further investigations into MTHFR function in B lymphoblasts.
