Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Comparing different methods for homocysteine determination.

Bruno Zappacosta1, Silvia Persichilli, Donata Scribano

  • 1Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Rome, Italy. b.zappacosta@uniserv.ccr.rm.cnr.it

Clinical Chemistry and Laboratory Medicine
|January 11, 2003
PubMed
Summary

Automated immunoassays offer a simpler way to measure homocysteine, a marker linked to cardiovascular disease. Three new automated methods showed strong correlation with the established HPLC technique, improving clinical lab efficiency.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Homologous recombination repair and breast cancer gene testing in prostate cancer: expert perspectives and practical guidance on best practice from sample acquisition to biomarker-informed clinical decision-making.

Frontiers in oncology·2026
Same author

Genomic profiling as an option for ovarian cancer diagnostics.

Expert review of molecular diagnostics·2026
Same author

Fertility-sparing treatment in stage IA grade 2 endometrioid endometrial cancer: Outcomes of a standardized approach.

Gynecologic oncology·2026
Same author

Fluoropyrimidine Cardiotoxicity: Role of Uridine Triacetate and Pharmacogenomic Insights from a Case of 5-FU-Induced Cardiogenic Shock.

Journal of cardiovascular pharmacology·2026
Same author

LANTERN 2: Association Between Gene Molecular Profile and STAS in Lung Adenocarcinoma: A Comparative Analysis in a Prospective Real-World Population.

Genes·2026
Same author

Enhanced Detection of BRCA Copy Number Alterations Within a Commercial HRD Assay: Implications for Precision Oncology in Ovarian Cancer.

International journal of molecular sciences·2026

Area of Science:

  • Clinical Chemistry
  • Biomarker Analysis
  • Cardiovascular Diagnostics

Background:

  • Elevated homocysteine levels are associated with thromboembolic diseases and can indicate metabolic or nutritional issues.
  • Past clinical use of homocysteine as a cardiovascular disease marker was hindered by complex measurement techniques and instrumentation.
  • Advancements in commercially available immunoassays now allow for simpler and faster homocysteine determination suitable for routine laboratories.

Purpose of the Study:

  • To evaluate the performance of three fully automated immunoassay methods for homocysteine determination.
  • To compare the results of these automated methods against the widely used High-Performance Liquid Chromatography (HPLC) technique.
  • To assess the suitability of automated immunoassays for routine clinical laboratory use in homocysteine measurement.

Related Experiment Videos

Main Methods:

  • Analysis of results from three fully automated homocysteine determination methods: Abbott IMx immunoassay, Abbott AxSYM immunoassay, and Immulite 2000 homocysteine immunoassay.
  • Comparison of the automated immunoassay results with those obtained using the established High-Performance Liquid Chromatography (HPLC) method.
  • Statistical correlation analysis including slope, intercept, and recovery rates.

Main Results:

  • All three automated immunochemical methods demonstrated excellent correlation with the HPLC method.
  • Correlation coefficients showed slopes between 0.97-1.03 and intercepts between 0.95-1.91.
  • Recovery rates for all three automated methods were consistently above 95%.

Conclusions:

  • The evaluated automated immunochemical methods for homocysteine determination are reliable and accurate.
  • These methods show strong correlation with the HPLC technique, validating their use in clinical settings.
  • The simplicity and speed of these automated immunoassays make them highly suitable for routine clinical laboratory practice.