Related Experiment Video

Updated: Sep 27, 2026

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
11:10

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Published on: August 11, 2014

The interplay between Salmonella typhimurium and its macrophage host--what can it teach us about innate immunity?

Sheena A Linehan1, David W Holden

  • 1The Department of Infectious Diseases, Centre for Molecular Microbiology and Infection, Imperial College of Science, Technology and Medicine, The Flowers Building, Armstrong Road, London SW7 2AZ, UK. s.linehan@ic.ac.uk

Immunology Letters
|January 16, 2003
PubMed

Insights

Salmonella Typhimurium evades macrophage defenses by controlling its vacuole and counteracting free radicals. This review explores novel mechanisms of Salmonella survival within host macrophages and innate immunity.

Area of Science:

  • Microbiology
  • Immunology
  • Cell Biology

Background:

  • Salmonella Typhimurium is a facultative intracellular pathogen causing systemic disease in mice.
  • Its virulence depends on survival and replication within macrophages.
  • Understanding Salmonella's interaction with macrophages is key to dissecting host-pathogen mechanisms.

Purpose of the Study:

  • Review recent studies on Salmonella-macrophage interplay.
  • Highlight mechanisms Salmonella uses to establish a permissive intracellular niche.
  • Identify host activities Salmonella must overcome for survival.

Main Methods:

  • Review of existing literature on Salmonella Typhimurium pathogenesis.
  • Analysis of studies focusing on Salmonella's intracellular survival strategies.
  • Examination of macrophage innate immune responses to Salmonella.

Main Results:

  • Salmonella maintains itself in a vacuole, avoiding macrophage cytosol.
  • The pathogen actively controls vacuole maturation, evading degradation.
  • Salmonella synthesizes compounds to counteract macrophage-generated free radicals, employing novel avoidance strategies.

Conclusions:

  • Salmonella employs sophisticated mechanisms to survive within macrophages.
  • These mechanisms involve vacuolar control and free radical neutralization.
  • Further research into these interactions reveals new aspects of macrophage cell biology and innate immunity.

More Related Videos

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages10:33

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Published on: October 25, 2017

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization09:25

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

Published on: July 28, 2015

Related Concept Videos

Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Immune Surveillance by NK Cells and Phagocytes01:27

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview
Colonisation of Pathogens01:25

Colonisation of Pathogens

Pathogen colonization of host tissues is a critical step in the development of infectious diseases. Various pathogenic microorganisms, including bacteria, fungi, viruses, and protozoa, have evolved complex strategies to attach to, invade, and persist within host environments. These mechanisms enable pathogens to establish infections, evade immune responses, and resist antimicrobial treatments.Attachment to Host CellsIn bacteria, colonization typically begins with adherence to host epithelial...
Introduction to Innate and Adaptive Immunity01:21

Introduction to Innate and Adaptive Immunity

The human immune system is a complex defense mechanism that protects the body from harmful pathogens and foreign substances. It comprises two crucial components: innate and adaptive immunity.
Innate immunity is the body's natural, nonspecific defense system that acts quickly to protect against pathogens. It incorporates physical barriers like skin and mucous membranes and cellular elements such as phagocytes and natural killer cells. This part of our immune system provides an immediate,...