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Related Experiment Videos

Granulocyte function in grancalcin-deficient mice.

Jürgen Roes1, B Ken Choi, David Power

  • 1Department of Immunology and Molecular Pathology, Windeyer Institute of Medical Sciences, University College London, London W1T 4JF, United Kingdom. j.roes@ucl.ac.uk

Molecular and Cellular Biology
|January 17, 2003
PubMed
Summary

Grancalcin (Gca) is a calcium-binding protein in neutrophils. Grancalcin-deficient mice show no essential role in immune cell functions or fighting infections, suggesting it

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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Grancalcin (Gca) is a penta-EF-hand calcium-binding protein highly expressed in neutrophils.
  • EF-hand proteins regulate crucial cellular processes like cell migration, apoptosis, and neutrophil effector functions.

Purpose of the Study:

  • To investigate the in vivo role of grancalcin in neutrophil function and host defense.
  • To determine if grancalcin is essential for leukocyte effector functions and resistance to infection.

Main Methods:

  • Gene targeting in embryonic stem cells to create grancalcin-deficient (Gca mutant) mice.
  • Assessment of leukocyte recruitment during induced inflammation.
  • Evaluation of resistance to systemic fungal infection and in vitro killing of Staphylococcus aureus.

Related Experiment Videos

  • Analysis of survival rates during endotoxic shock.

    • Main Results:

    • Grancalcin-deficient mice were healthy, reproduced normally, and showed no significant defects in leukocyte recruitment.
    • Mutant mice exhibited normal resistance to fungal infections and impaired Staphylococcus aureus killing by inflammatory cells.
    • Marginally increased survival in mutants during endotoxic shock suggests a potential role in immunopathogenesis.

    Conclusions:

    • Grancalcin is not essential for vital leukocyte effector functions required for controlling microbial infections.
    • The absence of grancalcin does not significantly impair host defense against fungal or bacterial challenges.
    • Grancalcin may play a minor role in the immunopathogenesis of endotoxic shock.