Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Flow cytometric basophil activation test: a review.

María L Sanz1, J P Maselli, P M Gamboa

  • 1Department of Allergology and Clinical Immunology, University Clinic of Navarra, School of Medicine, University of Navarra, Pamplona, Spain. mlsanzlar@unav.es

Journal of Investigational Allergology & Clinical Immunology
|January 18, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perspectives in Allergy Diagnosis.

International archives of allergy and immunology·2021
Same author

Nonsteroidal anti-inflammatory drug hypersensitivity syndrome: a multicenter study. II. Basophil activation by nonsteroidal anti-inflammatory drugs and its impact on pathogenesis.

Journal of investigational allergology & clinical immunology·2010
Same author

Nonsteroidal anti-inflammatory drug hypersensitivity syndrome. A multicenter study. I. Clinical findings and in vitro diagnosis.

Journal of investigational allergology & clinical immunology·2009
Same author

Pharmacovigilance of drug allergy and hypersensitivity using the ENDA-DAHD database and the GALEN platform. The Galenda project.

Allergy·2009
Same author

Cellular tests in the diagnosis of drug hypersensitivity.

Current pharmaceutical design·2008
Same author

Diagnostic tests based on human basophils: more potentials and perspectives than pitfalls.

International archives of allergy and immunology·2008

The basophil activation test (BAT), a flow cytometry method, reliably quantifies basophil activation for diagnosing allergies. This technique aids in identifying allergic and pseudoallergic reactions beyond IgE antibodies.

Area of Science:

  • Immunology
  • Cell Biology
  • Allergy Diagnostics

Background:

  • Flow cytometry analyzes cell characteristics using fluorescent markers.
  • The basophil activation test (BAT), or flow-cytometric allergen stimulation test (FAST), measures in vitro allergen-induced basophil activation.
  • This assay detects specific membrane protein markers expressed on activated basophils.

Purpose of the Study:

  • To review literature on BAT/FAST for immediate-type allergies (inhalant, drug, food).
  • To present own experience with this diagnostic technique.
  • To evaluate the diagnostic value of flow cytometry in allergy.

Main Methods:

  • Flow cytometry analysis of basophil activation markers.
  • In vitro stimulation of basophils with allergens.

Related Experiment Videos

  • Review of existing literature and presentation of clinical experience.

    • Main Results:

    • Flow cytometry provides reliable quantification of basophil activation in vitro and in vivo.
    • The technique can detect allergic and pseudoallergic reactions.
    • BAT/FAST shows promise for allergy diagnosis.

    Conclusions:

    • Flow cytometry is a reliable method for assessing basophil activation in allergy diagnostics.
    • BAT/FAST can identify reactions mediated by mechanisms other than IgE.
    • Further clinical evaluation is needed to establish the diagnostic value of this technique.