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Related Experiment Videos

Flow cytometric basophil activation test: a review.

María L Sanz1, J P Maselli, P M Gamboa

  • 1Department of Allergology and Clinical Immunology, University Clinic of Navarra, School of Medicine, University of Navarra, Pamplona, Spain. mlsanzlar@unav.es

Journal of Investigational Allergology & Clinical Immunology
|January 18, 2003
PubMed
Summary

The basophil activation test (BAT), a flow cytometry method, reliably quantifies basophil activation for diagnosing allergies. This technique aids in identifying allergic and pseudoallergic reactions beyond IgE antibodies.

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Area of Science:

  • Immunology
  • Cell Biology
  • Allergy Diagnostics

Background:

  • Flow cytometry analyzes cell characteristics using fluorescent markers.
  • The basophil activation test (BAT), or flow-cytometric allergen stimulation test (FAST), measures in vitro allergen-induced basophil activation.
  • This assay detects specific membrane protein markers expressed on activated basophils.

Purpose of the Study:

  • To review literature on BAT/FAST for immediate-type allergies (inhalant, drug, food).
  • To present own experience with this diagnostic technique.
  • To evaluate the diagnostic value of flow cytometry in allergy.

Main Methods:

  • Flow cytometry analysis of basophil activation markers.
  • In vitro stimulation of basophils with allergens.

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  • Review of existing literature and presentation of clinical experience.

    • Main Results:

    • Flow cytometry provides reliable quantification of basophil activation in vitro and in vivo.
    • The technique can detect allergic and pseudoallergic reactions.
    • BAT/FAST shows promise for allergy diagnosis.

    Conclusions:

    • Flow cytometry is a reliable method for assessing basophil activation in allergy diagnostics.
    • BAT/FAST can identify reactions mediated by mechanisms other than IgE.
    • Further clinical evaluation is needed to establish the diagnostic value of this technique.