Related Experiment Video
Updated: Sep 27, 2026

Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
Published on: May 4, 2011
pO(2) measurements by phosphorescence quenching: characteristics and applications of an automated system
Heinz Kerger1, Gesine Groth, Armin Kalenka
1Department of Anesthesiology, University Hospital of Mannheim, D-68135 Mannheim, Germany.
Abstract:
An automated system for pO(2) analysis based upon phosphorescence quenching was tested. The system was calibrated in vitro with capillary samples of saline and blood. Results were compared to a conventional measuring procedure wherein pO(2) was calculated off-line by computer fitting of phosphorescence decay signals. PO(2) measurements obtained by the automated system were correlated (r(2) = 0.98) with readings simultaneously generated by a blood gas analyzer, accuracy being highest in the low (0-20 mm Hg) and medium pO(2) ranges (21-70 mm Hg). Measurements in in vivo studies in the hamster skin-fold preparation were similar to previously reported results. The automated system fits the phosphorescence decay data to a single exponential and allows repeated pO(2) measurements in rapid sequence.
More Related Videos
Related Concept Videos
Photoluminescence: Applications
Fluorescence and Phosphorescence: Instrumentation
Gas Chromatography: Types of Detectors-II
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
Flame Photometry: Lab
Flame Photometry: Overview

