[Changes of renal blood flow and serum NO2-/NO3- in rabbits treated with mannitol at different dosages]

To observe the changes of renal blood flow and serum NO2-/NO3- in rabbits administrated with mannitol at different dosages, and investigate the mechanism of acute renal failure (ARF) induced by massive mannitol. Eighteen healthy male Zealand rabbits were randomly divided into 3 groups: minor mannitol group(n = 6, 8 g.kg-1 within 2 hours), control group (n = 6, saline of the same volume), massive mannitol group with free water taking(n = 6, mannitol 40-60 g.kg-1 with 3 days). The changes of renal function, renal blood flow and NO2-/NO3- in serum and urine were observed. The results showed that: no significant change in creatinine clearance (Ccr), renal blood flow (RBF) and serum NO2-/NO3- was found between minor mannitol group and control group. In massive mannitol group with free water taking, Ccr and RBF significantly decreased and serum NO2-/NO3- significantly increased (P > 0.05). The results suggest that ARF induced by massive mannitol is associated with a significant decline of RBF, on the other hand, endothelium-derived relaxing factor(EDRF) maybe compensatively increase.