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[Simultaneous determination of catecholamine levels in plasma by high performance liquid chromatography]
1Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China.
Objective:
To use a high performance liquid chromatography with electrochemical detection to simultaneously determine catecholamine(CA) levels in plasma and to diagnose chromaffin cell tumors and neuroblastoma.
Methods:
The plasma samples after flowing extraction by ion-moderated partition were determined with electrochemical method to detect CA levels in plasma; the lowest detective limit, the precision, recovery, sensitivity of CA levels were tested and a reference range was established based on the respective data of 18 healthy persons.
Results:
The recovery rates of epinephrine and norepinephrine were 86.2% and 90.5%. The method of testing epinephrine was linear at the range of 0.2 to 10.6 nmol.L-1 with 0.05 nmol.L-1 of the lowest detective limit. The intra- and inter-assay coefficients of variation were lower than 10.7% and 11.1%, respectively.
Conclusion:
The method is simple, accurate, sensible in the clinical diagnosis of CA levels.