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[The millipore rapid screening of hydantoinase production strains]
1Department of Biochemistry, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Hua Xi Yi Ke Da Xue Xue Bao = Journal of West China University of Medical Sciences = Huaxi Yike Daxue Xuebao
|January 23, 2003
Abstract
Objective:
To find a strain with a higher hydantoinase activity.
Methods:
The millipore rapid screening method was established to select the strains. The effects of several solvents on the rate of biotransformation were compared.
Results:
A hydantoinase production strain has been selected by the millipore rapid screening method. Tween-80 (1%) is found to be a suitable solvent to dissolve the substrate.
Conclusion:
A new method is provided for rapid selection of hydantoinase production strains from large-scale samples. The difficulty in dissolving the substrate has been resolved to some extent.