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A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
Published on: April 28, 2019
[Inhibitory effect of the combination of DHPAs and hyperbaric oxygen on Japanese B encephalitis virus infection in
1Department of Infectious Disease, Xiangya Hospital, Central South University, Changsha 410008, China.
Objective:
To investigate the inhibitory effect of the combination of 2,3-dihydroxypropyl-adenine (DHPAs) and hyperbaric oxygen (HBO) on Japanese B encephalitis virus infection in mice.
Methods:
Mice infected P3-55 and P3-53 virus were used in the experiments. Virus was inoculated intraperitoneally to Kumming strain mice (8.5-9.5 g). Mice were divided into groups and treated in the incubation period of the infection. The duration of treatment was 5 days, and the experiments were terminated at the end of 2 weeks after treatment. The living and the dead were then counted finally.
Results:
The intergroup comparison showed marked difference between the control group and combination of DHPAs and hyperbaric oxygen group (P < 0.025 and P < 0.01), but there was no statistical difference between other groups and the control group (P > 0.05).
Conclusion:
The combined treatment of (S) DHPA and/or (RS)-DHPA and HBO has inhibitory effect on Japanese encephalitis virus infection in experimental mice.
Insights
The combination of 2,3-dihydroxypropyl-adenine (DHPA) and hyperbaric oxygen (HBO) significantly inhibited Japanese encephalitis virus infection in mice. This combined therapy shows promise for treating viral encephalitis.
Area of Science:
- Virology
- Infectious Diseases
- Hyperbaric Medicine
Background:
- Japanese encephalitis virus (JEV) is a significant cause of viral encephalitis globally.
- Limited effective treatments exist for JEV infection.
- Investigating novel therapeutic strategies is crucial.
Purpose of the Study:
- To evaluate the inhibitory effect of combining 2,3-dihydroxypropyl-adenine (DHPA) with hyperbaric oxygen (HBO) on JEV infection.
- To assess the efficacy of this combined treatment in a murine model.
Main Methods:
- Kumming strain mice were infected with JEV.
- Mice were treated with DHPA and/or HBO during the incubation period for 5 days.
- Survival rates were monitored for two weeks post-treatment.
Main Results:
- The combination of DHPA and HBO demonstrated a statistically significant difference compared to the control group (P < 0.025 and P < 0.01).
- Other treatment groups did not show a significant difference compared to the control (P > 0.05).
Conclusions:
- Combined DHPA and HBO therapy exhibits an inhibitory effect on Japanese encephalitis virus infection in experimental mice.
- This combination therapy represents a potential therapeutic approach for JEV infections.
