[Separation and determination of three plant internal hormones by gas chromatography]

A method for the simultaneous separation and determination of three plant internal hormones indole acetic acid (IAA), abscisic acid(ABA) and gibberellic acid(GA) in plant by gas chromatography with wide-bore capillary column is described. The gas chromatographic conditions were as follows: FID detector; HP-1 capillary column, 5 m x 0.53 mm x 2.65 microns film; the column temperature, 220 degrees C; the injector temperature, 250 degrees C; the detector temperature, 280 degrees C, carrier gas, 3.5 mL/min N2; internal standard n-docosane. All components and internal standard were separated in 8 min. The detection limit of IAA, ABA and GA were 0.16, 0.08 and 0.48 mg/L respectively. The relative standard deviations were 2.2%, 1.7% and 2.8% respectively. The linear range were 0.16-80 mg/L(r = 0.9986), 0.08-40 mg/L (r = 0.9993) and 0.48-240 mg/L (r = 0.9991) respectively. The average recoveries were (88.4 +/- 2.4%)%, (92.2 +/- 1.2)% and (91.8 +/- 1.8)% respectively. The method is simple, rapid, sensitive and reproducible.