Related Experiment Video
Updated: Sep 27, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
[Determination of phenols in environmental water with bromination derivatization and gas chromatography]
Abstract:
A method for the determination of phenols in environmental water with bromination derivatization and gas chromatography is described. The phenols were transformed to bromophenols and then extracted with toluene and separated by the FFAP wide-bore capillary column and detected by electron capture detector (ECD). The results showed that the linear ranges were 0.06-80 micrograms/L for o-methylphenol and p-methylphenol, 0.03-40 micrograms/L for phenol and m-methylphenol. The detection limits for o-methylphenol, p-methylphenol, phenol and m-methylphenol were 0.03, 0.06, 0.01, 0.015 microgram/L respectively. The method is simple, rapid and reliable for determining phenols in environmental water.
Related Concept Videos
Halogenation of Alkenes
Consider the bromination of cyclopentene. Molecular bromine is polarized in the proximity of the π electrons of cyclopentene. An electrophilic bromine atom adds across the double bond, forming a cyclic bromonium ion intermediate.
Formation of Halohydrin from Alkenes
Hydrolysis of Chlorobenzene to Phenol: Dow Process
Reactions at the Benzylic Position: Halogenation
Electrophilic Aromatic Substitution: Chlorination and Bromination of Benzene
Gas Chromatography: Types of Detectors-II

