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Published on: December 16, 2022
[Determination of the adenosine triphosphate in myocardial tissue by ion-pair reversed-phase high performance liquid
1Fu Wai Hospital for Cardiology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China.
Abstract:
ATP depletion is an important factor contributing to the incomplete recovery of ventricular function following ischemia and reperfusion. A simple and rapid method for determination of adenosine triphosphate(ATP) in myocardial tissue by RP-HPLC was developed. The chromatographic conditions were as follows: Spherisorb ODS2 column, V(40 mmol/L KH2PO4 and 5 mmol/L TBAP):V(methanol) = 74:26 (pH 6.2) mobile phase and UV detector at 259 nm. The ATP in myocardium was extracted with 0.4 mol/L HClO4. The calibration curve showed a good linearity in 5 mg/L-100 mg/L(r = 0.9998). The recovery ranged from 97.8% to 104.1%. The limit of detection was 2 mg/L. The verified results demonstrated that this method is precise, accurate and can be used for determination of ATP in myocardial tissue.
