[Determination of activity of bone alkaline phosphatase in whole serum by high performance liquid chromatography]

An analytical method has been established for the determination of bone alkaline phosphatase (BALP) activity using high performance liquid chromatography(HPLC). The stainless steel column was 22 cm x 4.6 mm i.d. packed with totally porous, spherical silica particles. A solution of methanol and 0.01 mol/L CH3COOH(30/70, V/V) adjusted to pH 4.8 was employed as the mobile phase. The flow rate was 0.8 mL/min. Chromatography was performed with ultraviolet detector at 275 nm. Linear calibration curve for BALP was measured within the range of 8-200 mg/L with correlation coefficient of 0.996. The lowest detection limit was 3 ng. The HPLC method described is simple, rapid and reliable and suitable for clinical monitoring.