[Study on the direct determination of auxins by gas chromatography]

A method for the direct determination of auxins, indole acetic acid (IAA), indole-butyric acid (IBA) and naphthylacetic acid (NAA), by gas chromatography with wide-bore capillary column is described. The gas chromatographic conditions were as follows: HP-1 capillary column, 5 m x 0.53 mm i.d., 2.65 microns film, the column temperature 180 degrees C, the temperature of injector 250 degrees C, the temperature of FID detector 260 degrees C, carrier gas: N2 20 mL/min and dibutyl phthalate was used as internal standard. All components and internal standard were separated in 3 min. The relative standard deviations were from 0.61% to 1.14%. The method is simple, rapid, sensitive, good reproducible and satisfactory.