[The preliminary study on the open tubular capillary affinity liquid chromatography]

A novel mode of affinity chromatography based on the open tubular column (OTAC) is presented in this paper. The OTAC column was prepared by coating a layer of Cibacron Blue F3GA onto the inner surface of capillary column with 50 microns inner diameter. The affinity chromatography experiment was performed on a capillary electrophoresis instrument by using its pressure system as driving force. The bovine serum albumin (BSA) and lysozyme (Lys) were successfully separated with step gradient elution. The relative standard deviation (RSD) for 10 consecutive runs for the elution time of lysozyme was less than 0.1%, and those for its peak area and peak height were 4.3% and 3.7% respectively. The good linear relationships of the amount of the injected lysozyme versus its peak area and peak height were observed with correlation coefficients of 0.9946 and 0.9988 respectively in the range from 8.6 ng to 28.7 ng.