[Determination of degraded products of penicillin by high performance liquid chromatography]
1Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. zhaomei@dicp.ac.cn
Abstract:
A method for the determination of main composition in degraded products of penicillin, 6-aminopenicillanic acid (6-APA), penicillin G kalium (PGK), phenylacetic acid (PAA), and the by-product benzylpenicilloic acid (BPA) by HPLC was studied. Separation conditions were as follows: Spherisorb C18 column, 250 mm x 4.6 mm i.d., 10 micrograms; mobile phase: V (methanol): V(0.004 mol/L KH2PO4 buffer, pH 4.5) = 50:50; flow rate: 1 mL/min; detector: UV 230 nm. The method is effective, quick, accurate and reproducible. The satisfactory results show that this new method has certain practical values as an approach of quality control.
More Related Videos
Related Concept Videos
Production of Antibiotics
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process


