[High performance liquid chromatographic determination of internal hormones in inter-subspecific hybrid rice]

A simple, rapid and perfect extraction and determination method for the internal hormones (gibberellin acid (GA3), indole-3-acetic acid (IAA), zeatin (Z), abscisic acid (ABA)) in inter-subspecific hybrid rice with reversed-phase high performance liquid chromatography has been developed. The chromatographic conditions were as follows: a Waters C18 column (4.6 mm i.d. x 250 mm, 5 microns), an SPD-6AV detector set at 254 nm and the mobile phase was a mixture of methanol, water and acetic acid (45:54.2:0.8, volume ratio). The lower detection limits were 0.5 mg/L for GA3, 0.1 mg/L for IAA, 0.3 mg/L for Z and 0.03 mg/L for ABA.