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Updated: Jul 10, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Major histocompatibility complex class II DOA sequences from three Antarctic seal species verify stabilizing
D J Decker1, B S Stewart, N Lehman
1Department of Biology, Portland State University, Portland, OR 97207, USA.
Abstract:
To provide additional support for the sequence conservation and hence the regulatory role of the MHC class II DOA locus, we obtained the nucleotide sequences of exon 2 and exon 3, along with the intervening intron, of the Ross seal, and sequences from the exon 2 region from the Weddell and leopard seals. These are the first reports of the sequences of this locus from a carnivore species. The results demonstrate strong conservation among mammals for the exon sequence and produce a gene genealogy that is consistent in topology with a species tree.
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