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Reciprocal action between BMP-2 and BMP-3 in cultured fibroblast in vitro
Guo-lin Meng1, Yun-yu Hu, Qin Pu
1Orthopaedic Institute of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. mengguolin@163.net
Objective:
To explore reciprocal action between BMP-2 (bone morphogenetic protein-2) and BMP-3 for better understanding of the mechanism of BMP during bone fracture union.
Methods:
rhBMP-2 was added into the cultured fibroblasts with the concentration of 1,200 ng/ml. The expression of BMP-3 in fibroblasts was detected by immunohistochemistry. Eukaryotic expression vector pcDNA3-BMP-3 was transfected into the fibroblasts. After the effective expression of BMP-3 was identified, BMP-2 was also detected by immunohistochemistry in BMP-3 expression cells. The fibroblasts transfected with empty vector pcDNA3 were used as the control.
Results:
Exogenous rhBMP-2 could promote the expression of BMP-3 in fibroblasts. BMP-3 also could be detected in these cells.
Conclusions:
BMP-2 and BMP-3 could reciprocally adjust the expression in fibroblasts.